Methylation enrichment pyrosequencing: combining the specificity of MSP with validation by pyrosequencing

Methylation enrichment pyrosequencing: combining the specificity of MSP with validation by pyrosequencing

It has been suggested that detection of aberrant DNA methylation in clinical specimens such as sputum or saliva may be a valuable tumour biomarker. Any clinically applicable detection technique must combine high sensitivity with high specificity. In this study we describe methylation enrichment pyrosequencing (MEP), which benefits from the high sensitivity and specificity of methylation-specifi...

Serial pyrosequencing for quantitative DNA methylation analysis.

BioTechniques 721 In mammals, DNA methylation is a genetically programmed DNA modification and occurs almost exclusively at the 5-position of the pyrimidine ring of cytosines. Cytosine methylation is essential for mammalian embryogenesis and development, implicated in X inactivation, and influences gene expression of the corresponding genes, giving rise to distinct expressions patterns in diffe...

CpG Methylation Analysis by Pyrosequencing Benchmarks and Application

Investigation of MGMT and DAPK1 methylation patterns in diffuse large B-cell lymphoma using allelic MSP-pyrosequencing

The tumor suppressor genes MGMT and DAPK1 become methylated in several cancers including diffuse large B-cell lymphoma (DLBCL). However, allelic methylation patterns have not been investigated in DLBCL. We developed a fast and cost-efficient method for the analysis of allelic methylation based on pyrosequencing of methylation specific PCR (MSP) products including a SNP. Allelic methylation patt...

DNA Methylation of GSTP1 in Human Prostate Tissues: Pyrosequencing Analysis

PURPOSE DNA methylation is an important epigenetic mechanism of gene regulation and plays essential roles in tumor initiation and progression. Differences in methylation patterns between neoplastic and normal cells can be used to detect the presence of cancer. The aim of the present study was to evaluate the usefulness of glutathione-S-transferase-Pi (GSTP1) hypermethylation in discriminating b...